dmba as a carcinogenic agent in research
Millions are diagnosed with cancer worldwide, each year. Unraveling the early events in cancer can best be achieved by using animal models. The establishment of animal models for oral cancer has been thoroughly attempted for decades.12-dimethylbenz(a)anthracene (DMBA) is a known carcinogen that is widely used in many cancer models.
This study aimed to evaluate the effect of DMBA on different oral tissue of rat models.
Materials and methods:
The rats were divided into four groups. Control group (group I) and three experimental groups. The experimental groups were divided according to the studied tissue into, group II: salivary glands, group III: tongue mucosa and group IV: buccal mucousa. Experimental groups received DMBA emulsion via gastric gavage as a single weekly dose for 6 weeks.
Histopathologically, H&E assessment revealed architectural changes of the tissues along with inflammation and hyperplasia among other histopathological features. IHC with KI 67 exhibited no significant differences in expression between the studied groups.
DMBA for 6 weeks gastric gavage failed to form tumor masses but caused histopathological alterations.